Coding

Part:BBa_K3812000

Designed by: Guanghui Dong   Group: iGEM21_CHINA-FAFU   (2021-09-30)


Code Ascorbate peroxidase (AP)

BBa_K3812000 can encode APX1.APX is an important enzyme component in the AsA-GSH cycle in plant cells. AsA is used as an electron donor to convert H2O2 to H2O. At the same time, As A is oxidized, it can be regenerated through recycling. The proper concentration of endogenous As A is critical to the stability of APX. According to cell location, plant APX has several isoenzymes, namely: ① cytoplasmic APX (c APX), mainly APX1; ② chloroplast APX, including matrix type (s APX) and thylakoid type (t APX); ③ mitochondria APX (mitAPX); ④Micro body APX (m APX). Among them, APX1 located in the cytoplasm plays a key role in regulating the level of H2O2 and its signal transduction, and occupies a core position in the ROS scavenging network.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 748
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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